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Histidine-tagged Protein Purification And DetectionIMAC = Immobilized Metal Ion Affinity Chromatography, IEX = Ion Exchange Chromatography, SEC = Size Exclusion Chromatography, TAG Cleavage Can Be Performed Either After The IMAC Step Or While The Tagged Protein Is Still Bound To The IMAC Resin/column. 1-step Protocol Manual Use Or System (ÄKTA Start/ÄKTA Pure) 2-step Protocol System Recommended 4th, 2024Ni-NTA Resin, Nickel IMAC Resin For 6X His Tagged Protein ...Desalting Columns) Or Dialysis (G-Biosciences Tube-O- ... Column Regeneration Should Be Performed When A Different Protein Is Being Isolated Or When There Is A Significant Loss In The Yield Of Protein. If The Ni-NTA Resin Loses Its Blue Color The Column Needs Recharging. 2th, 2024For Manual And Automated Assays Using 6xHis-tagged ProteinsHandbook For Manual And Automated Assays Using 6xHis-tagged Proteins Purification Of 6xHis-tagged Proteins Second Edition ... Actions, And Immunoassay Procedures Requiring Higher Binding Capacities For The Captured Molecule Than Achieve 1th, 2024.
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